Use of ELISA to Monitor Bacterial Kidney Disease in Naturally Spawning Chinook Salmon - Information Reports 2006

Identification Information

Citation

Originator: O’Conner, G. O. T. L. Hoffnagle

Publish Date: 2006

Online Link: https://www.fws.gov/lsnakecomplan/Reports/ODFWreports.html

BPA Project #:

Contact Information

Agency: Oregon Department of Fish and Wildlife

Name: Joseph Feldhaus

Job Position: Assistant Project Leader

Telephone: 541-962-3724

E-Mail Address: Joseph.Feldhaus@odfw.oregon.gov

Description

Abstract: Bacterial kidney disease (BKD), caused by Renibacterium salmoninarum (Rs), is a serious problem among Pacific Northwest salmon hatcheries, including the concern that salmon reared in hatcheries may spread BKD to natural populations. In order to monitor the potential spread of this disease to salmon spawning in nature, a method must be available to collect and analyze tissues from naturally spawning salmon. Kidney tissue analyzed by enzyme-linked immunosorbent assay (ELISA) is the standard method to detect the presence of Rs in salmon sampled in hatcheries. We tested the ability to use ELISA on kidney tissue collected from intact carcasses recovered on the spawning grounds to monitor BKD in naturally spawning populations. We compared ELISA optical density (OD) values from kidney tissue that was subjected to simulated conditions to which kidney tissue would be exposed in a decomposing carcass and following collection during a spawning ground survey versus samples collected from freshly killed salmon at a hatchery. Mean ELISA OD levels were 1.060 for the samples prepared by the normal preparation and 1.115 for samples prepared by simulating spawning ground survey collection. We found no significant difference in mean ELISA optical density between the sample preparations and the relationship between them was nearly 1:1 (slope = 0.946). This demonstrates that BKD prevalence in natural populations can be monitored using ELISA conducted on samples from intact carcasses on spawning ground surveys. This will be an important tool for monitoring the effect of hatchery supplementation on naturally spawning salmon populations. Lower Snake River Compensation Plan (LSRCP) ODFW- Eastern Oregon Fish Research (EOFR)

Purpose: ODFW Information Report

Time Period of Content: 2005

Geographic Extent: Grande Ronde River basin

Status: Final

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Format: pdf

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